The Basic Principles Of Ordre mondial
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If creating v2 sample sheets for instruments in addition to the ones listed, confirm the final sample sheet has the i5 index in the correct orientation as explained above.
Index 1: the i7 index column is within the ahead orientation (match the orientation if the i7 indexes in the sample sheet).
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When demultiplexing, the ultimate sample sheet provided for the FASTQ era program needs to possess the i5 index in the right orientation for that System. On the other hand, most Illumina sample sheet technology program handles this immediately.
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When evaluating index sets for color harmony, the sequence observed because of the instrument should be assessed, whatever the orientation useful for the sample sheet. For your i7 index, normally asses colour equilibrium using the "i7 bases for sample sheet" column orientation.
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